Application
Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-LIN28 antibody ()Application
WB Suggested Anti-LIN28 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Hela cell lysate| Product Name | Rabbit anti-LIN28 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CSDD1, FLJ12457, LIN-28, LIN28A, ZCCHC1, LIN28, lin-28A |
| Immunogen | The immunogen for anti-LIN28 antibody: synthetic peptide directed towards the N terminal of human LIN28 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogPigRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIN28 |
|---|---|
| Gene Synonyms | LIN28A |
| Gene Full Name | Lin-28 homolog A (C. elegans) |
| Gene Summary | LIN28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. The association of LIN28 with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. IT binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. LIN28 is Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. |
| Alternative Names | CSDD1 FLJ12457 LIN-28 LIN28A ZCCHC1 LIN28 lin-28A |
| Molecular Weight(MW) | 23kDa |
| Sequence | 209 amino acids |
Application
Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-LIN28 antibody ()
Application
WB Suggested Anti-LIN28 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
