Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Product Name | LIN28 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIN28, CSDD1, FLJ12457, LIN-28, LIN28A, ZCCHC1, LIN28, lin-28A |
| Product description | LIN28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. The association of LIN28 with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. IT binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. LIN28 is Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression.1) Gregory, S.G., (2006) Nature 441 (7091), 315-321. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LIN28. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| LIN28 antibody can be used for detection of LIN28 by ELISA at 1:62500. LIN28 antibody can be used for detection of LIN28 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIN28A |
|---|---|
| Alternative Names | LIN28 CSDD1 FLJ12457 LIN-28 LIN28A ZCCHC1 LIN28 lin-28A |
| Molecular Weight(MW) | 23 kDa |
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | LIN28 antibody can be used for detection of LIN28 by ELISA at 1:62500. LIN28 antibody can be used for detection of LIN28 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store LIN28 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
