Application
Immunofluorescence of LIN28 in human spleen tissue with LIN28 antibody at 20 μg/mL.Application
Immunohistochemistry of KLF4 in human spleen tissue with KLF4 antibody at 5 μg/mL.Application
Western blot analysis of Lin28 in Raji cell lysate with Lin28 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Lin28 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lin28 Antibody: CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A, CSDD1, Protein lin-28 homolog A, Zinc finger CCHC domain-containing protein 1, Lin-28A |
| Product description | Lin28 Antibody: Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.1) Ambros V. A hierarchy of regulatory genes controls a larva-to-adult developmental switch in C. elegans. Cell1989; 57:49-57. |
| Immunogen | Lin28 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Lin28. The immunogen is located within the last 50 amino acids of Lin28. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Lin28 antibody can be used for detection of Lin28 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIN28 |
|---|---|
| Alternative Names | Lin28 Antibody: CSDD1 LIN28 LIN-28 ZCCHC1 lin-28A CSDD1 Protein lin-28 homolog A Zinc finger CCHC domain-containing protein 1 Lin-28A |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunofluorescence of LIN28 in human spleen tissue with LIN28 antibody at 20 μg/mL.
Application
Immunohistochemistry of KLF4 in human spleen tissue with KLF4 antibody at 5 μg/mL.
Application
Western blot analysis of Lin28 in Raji cell lysate with Lin28 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | Lin28 antibody can be used for detection of Lin28 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Lin28 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Lin28 Antibody is supplied in PBS containing 0.02% sodium azide. |
