Immunohistochemical
ImmunohistochemicalWestern blot
Host: Rabbit Target Name:ILF2 Sample Tissue:JurkatLane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%Western blot
Sample Type: MCF-7 WT a whole cell lysates (110UG) Primary Dilution: 1.5mg/mL Secondary Antibody: LI-COR Donkey anti-rabbitSecondary Dilution: 1:10,000Western blot
1.25ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-ILF2 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NF45, PRO3063 |
| Immunogen | The immunogen for anti-ILF2 antibody: synthetic peptide directed towards the C terminal of human ILF2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ILF2 |
|---|---|
| Gene Synonyms | NF45 PRO3063 |
| Gene Full Name | Interleukin enhancer binding factor 2, 45kDa |
| Gene Summary | Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of the ILF2 gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. |
| Alternative Names | NF45 PRO3063 |
| Molecular Weight(MW) | 43kDa |
| Sequence | 390 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
Host: Rabbit Target Name:ILF2 Sample Tissue:JurkatLane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%
Western blot
Sample Type: MCF-7 WT a whole cell lysates (110UG) Primary Dilution: 1.5mg/mL Secondary Antibody: LI-COR Donkey anti-rabbitSecondary Dilution: 1:10,000
Western blot
1.25ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
