Application
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.Application
Antibody used in WB on Human MCF-7 at 1.5mg/mL.Application
Antibody used in IHC on Human kidney.| Product Name | ILF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ILF2, NF45, PRO3063 |
| Product description | Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of the ILF2 gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.1) Warner C. Greene, et al., (2006) Proc Natl Acad Sci 103:15588. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ILF2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| ILF2 antibody can be used for detection of ILF2 by ELISA at 1:62500. ILF2 antibody can be used for detection of ILF2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ILF2 |
|---|---|
| Alternative Names | ILF2 NF45 PRO3063 |
| Molecular Weight(MW) | 43 kDa |
Application
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Application
Antibody used in WB on Human MCF-7 at 1.5mg/mL.
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ILF2 antibody can be used for detection of ILF2 by ELISA at 1:62500. ILF2 antibody can be used for detection of ILF2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ILF2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
