Western blot
0.6ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-ZP3 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZPC, ZP3A, ZP3B, Zp-3 |
| Immunogen | The immunogen for anti-ZP3 antibody: synthetic peptide directed towards the C terminal of human ZP3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanRabbit |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ZP3 |
|---|---|
| Gene Synonyms | ZPC ZP3A ZP3B Zp-3 |
| Gene Full Name | Zona pellucida glycoprotein 3 (sperm receptor) |
| Gene Summary | The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene |
| Alternative Names | ZPC ZP3A ZP3B Zp-3 |
| Molecular Weight(MW) | 47kDa |
| Sequence | 424 amino acids |
Western blot
0.6ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
