Application
Antibody used in WB on Human HepG2 at 0.6 ug/ml.| Product Name | ZP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZP3, ZPC, ZP3A, ZP3B, Zp-3 |
| Product description | The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene1) Chamberlin, M.E., et al., (1990) Proc. Natl. Acad. Sci. U.S.A. 87 (16), 6014-6018. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZP3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ZP3 antibody can be used for detection of ZP3 by ELISA at 1:62500. ZP3 antibody can be used for detection of ZP3 by western blot at 0.6 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ZP3 |
|---|---|
| Alternative Names | ZP3 ZPC ZP3A ZP3B Zp-3 |
| Molecular Weight(MW) | 47 kDa, 23 kDa |
Application
Antibody used in WB on Human HepG2 at 0.6 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ZP3 antibody can be used for detection of ZP3 by ELISA at 1:62500. ZP3 antibody can be used for detection of ZP3 by western blot at 0.6 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZP3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
