Western blot
Lane 1: 20ug mouse mesenchymal stem cell lysate Primary Antibody Dilution : 1:2000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:10,000 Color/Signal Descriptions:Dnmt1 Gene Name:Anonymous Submitted by:Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Mouse Lung| Product Name | Rabbit anti-Dnmt1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cxxc9, Dnmt, Dnmt1o, MTase, Met-1, Met1, MommeD2, MCMT, m.MmuI |
| Immunogen | The immunogen for anti-Dnmt1 antibody: synthetic peptide directed towards the N terminal of mouse Dnmt1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Dnmt1 |
|---|---|
| Gene Synonyms | Cxxc9 Dnmt Dnmt1o MTase Met-1 Met1 MommeD2 MCMT m.MmuI |
| Gene Full Name | DNA methyltransferase (cytosine-5) 1 |
| Gene Summary | Dnmt1 methylates CpG residues.Dnmt1 preferentially methylates hemimethylated DNA. Dnmt1 associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance.Dnmt1 associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones.Dnmt1 mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, Dnmt1 is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. |
| Alternative Names | Cxxc9 Dnmt Dnmt1o MTase Met-1 Met1 MommeD2 MCMT m.MmuI |
| Molecular Weight(MW) | 183kDa |
| Sequence | 1619 amino acids |
Western blot
Lane 1: 20ug mouse mesenchymal stem cell lysate Primary Antibody Dilution : 1:2000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:10,000 Color/Signal Descriptions:Dnmt1 Gene Name:Anonymous Submitted by:
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Mouse Lung| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
