Application
Western Blot analysis of human DNMT1 expression in nuclear extracts of HeLa cells (50 ug/lane) using rabbit anti Human DNMT1 at the indicated concentrations| Product Name | DNMT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DNMT1Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt 1, Dnmt 2 and Dnmt 3. Dnmt 1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt 2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt 3a and Dnmt 3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F 1, and HDAC 1. Dnmt 1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. |
| Immunogen | A synthetic peptide which represents a portion of human Dnmt1 encoded within exon 25. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: Use at a concentration of 2 - 4 μg/mg. WB: Use at a dilution of 1/1000 - 1/10,000. Predicted molecular weight: 153.6 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Dnmt1 P26358 DNMT CXXC9 126375 MCMT 1786 MGC104992 |
|---|---|
| Target | Dnmt1 |
| Molecular Weight(MW) | 153.6 kDa (note) |
| Cellular Localization | Nuclear |
Application
Western Blot analysis of human DNMT1 expression in nuclear extracts of HeLa cells (50 ug/lane) using rabbit anti Human DNMT1 at the indicated concentrations| Application Notes | IP: Use at a concentration of 2 - 4 μg/mg. WB: Use at a dilution of 1/1000 - 1/10,000. Predicted molecular weight: 153.6 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Tris-citrate/phosphate. pH 7-8 |
