Immunoblot
Lane 1: 7ug HEK293 cytoplasmic lysate 2: 7ug HEK293 nuclei lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: PARNSubmitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto UniversityWestern blot
1.25ug/mlELISA Titer: 1:312500Positive Control: SP2/0 cell lysate| Product Name | Rabbit anti-PARN polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DAN, 1200003I18Rik |
| Immunogen | The immunogen for anti-PARN antibody: synthetic peptide corresponding to a region of Mouse |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PARN |
|---|---|
| Gene Synonyms | DAN 1200003I18Rik |
| Gene Full Name | Poly(A)-specific ribonuclease (deadenylation nuclease) |
| Gene Summary | PARN is an oligomeric, processive, and cap-interacting 3' exonuclease. |
| Alternative Names | DAN 1200003I18Rik |
| Molecular Weight(MW) | 69kDa |
| Sequence | 624 amino acids |
Immunoblot
Lane 1: 7ug HEK293 cytoplasmic lysate 2: 7ug HEK293 nuclei lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: PARNSubmitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto University
Western blot
1.25ug/mlELISA Titer: 1:312500Positive Control: SP2/0 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
