PARN is an oligomeric, processive, and cap-interacting 3' exonuclease.1) Katayama, S., et al., (2005) Science 309 (5740), 1564-1566.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PARN.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PARN antibody can be used for detection of PARN by ELISA at 1:312500. PARN antibody can be used for detection of PARN by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HEK-293 at: 1:500 (Lane 1: 7ug HEK293 cytoplasmic lysate Lane 2: 7ug HEK293 nuclei lysate).
Application
Antibody used in WB on Mouse SP2/0 at 1.25 ug/ml.
Positive Control
1) SP2/0 Cell Lysate
Application Notes
PARN antibody can be used for detection of PARN by ELISA at 1:312500. PARN antibody can be used for detection of PARN by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PARN antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt