Western blot
2.5ug/ml
Positive Control: HepG2 cell lysate
WB
(B) Western blotting of LRH-1 protein in granulosa cells by treatment with various concentrations of DLPC.
IHC
(A) Immunochemistry assay was performed to visualize the localization of LRH-1 in bovine ovarian cells. Immuno-specific staining was brown, indicating LRH-1-positive cells. Immunohistochemistry was performed on three different ovarian slides from each of three bovines. Atretic follicles were indicated with the red arrow, whereas healthy follicles were indicated with black arrows. Bar: 200 μm.