Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Product Name | NR5A2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR5A2, B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, hB1F, hB1F-2, LRH1 |
| Product description | NR5A2 binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. It may be responsable for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. It is a key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. It may also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.1) Lee, Y.K., (2006) J. Biol. Chem. 281 (12), 7850-7855. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR5A2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NR5A2 antibody can be used for detection of NR5A2 by ELISA at 1:12500. NR5A2 antibody can be used for detection of NR5A2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NR5A2 |
|---|---|
| Alternative Names | NR5A2 B1F B1F2 CPF FTF FTZ-F1 FTZ-F1beta LRH-1 hB1F hB1F-2 LRH1 |
| Molecular Weight(MW) | 61 kDa, 56 kDa |
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NR5A2 antibody can be used for detection of NR5A2 by ELISA at 1:12500. NR5A2 antibody can be used for detection of NR5A2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR5A2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
