Western blot
2.5ug/mlPositive Control: Jurkat cell lysateImmunoblot
1. 6 ug mouse liver, heart and brain mitochondria extract 2. 6 ug mouse liver, heart and brain mitochondria extract 3. 6 ug mouse liver, heart and brain mitochondria extractPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: FECHSubmitted by: Dr. Hao Zhu, University of Kansas Medical Cente| Product Name | Rabbit anti-FECH polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EPP, FCE |
| Immunogen | The immunogen for anti-FECH antibody: synthetic peptide directed towards the N terminal of human FECH |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FECH |
|---|---|
| Gene Synonyms | EPP FCE |
| Gene Full Name | Ferrochelatase |
| Gene Summary | Ferrochelatase is localized to the mitochondrion where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Defects in ferrochelatase are associated with protoporphyria.Ferrochelatase is localized to the mitochondrion where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Defects in ferrochelatase are associated with protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | EPP FCE |
| Molecular Weight(MW) | 47kDa |
| Sequence | 429 amino acids |
| Entrez Gene | 2235 |
|---|---|
| SwissProt ID | Q8NAN0 |
| Protein Accession | NP_001012533 |
Western blot
2.5ug/mlPositive Control: Jurkat cell lysate
Immunoblot
1. 6 ug mouse liver, heart and brain mitochondria extract 2. 6 ug mouse liver, heart and brain mitochondria extract 3. 6 ug mouse liver, heart and brain mitochondria extractPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: FECHSubmitted by: Dr. Hao Zhu, University of Kansas Medical Cente| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
