Application
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.Application
Antibody used in WB on Mouse liver, heart and brain mitochondria at: 1:500 (Lanes: 1. 6 ug mouse liver, heart and brain mitochondria extract 2. 6 ug mouse liver, heart and brain mitochondria extract 3. 6 ug mouse liver, heart and brain mitochondria extract).| Product Name | FECH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Ferrochelatase is localized to the mitochondrion where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Defects in ferrochelatase are associated with protoporphyria.Ferrochelatase is localized to the mitochondrion where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Defects in ferrochelatase are associated with protoporphyria. Two transcript variants encoding different isoforms have been found for this gene.1) Maruyama, K. (2005) Blood 106 (3), 1098-1104. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FECH. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| FECH antibody can be used for detection of FECH by ELISA at 1:1562500. FECH antibody can be used for detection of FECH by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FECH |
|---|---|
| Alternative Names | FECH EPP FCE |
| Molecular Weight(MW) | 47 kDa, 48 kDa |
| Entrez Gene | 2235 |
|---|---|
| Protein Accession | NP_001012533 |
Application
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.
Application
Antibody used in WB on Mouse liver, heart and brain mitochondria at: 1:500 (Lanes: 1. 6 ug mouse liver, heart and brain mitochondria extract 2. 6 ug mouse liver, heart and brain mitochondria extract 3. 6 ug mouse liver, heart and brain mitochondria extract).| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | FECH antibody can be used for detection of FECH by ELISA at 1:1562500. FECH antibody can be used for detection of FECH by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store FECH antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
