Immunoblot
1. 6 ug mouse liver, heart and brain mitochondria extract 2. 6 ug mouse liver, heart and brain mitochondria extract 3. 6 ug mouse liver, heart and brain mitochondria extractPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: FECHSubmitted by: Dr. Hao Zhu, University of Kansas Medical CenteWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Product Name | Rabbit anti-FECH polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EPP, FCE |
| Immunogen | The immunogen for anti-FECH antibody: synthetic peptide directed towards the middle region of human FECH |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FECH |
|---|---|
| Gene Synonyms | EPP FCE |
| Gene Full Name | Ferrochelatase |
| Gene Summary | FECH is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene.The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | EPP FCE |
| Molecular Weight(MW) | 42kDa |
| Sequence | 423 amino acids |
Immunoblot
1. 6 ug mouse liver, heart and brain mitochondria extract 2. 6 ug mouse liver, heart and brain mitochondria extract 3. 6 ug mouse liver, heart and brain mitochondria extractPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: FECHSubmitted by: Dr. Hao Zhu, University of Kansas Medical Cente
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
