Immunohistochemical
ImmunohistochemicalWestern blot
0.25ug/mlPositive Control: HepG2 cell lysate| Product Name | Rabbit anti-GAPVD1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZP434C212, KIAA1521, MGC138847, MGC138848, RAP6, GAPEX5 |
| Immunogen | The immunogen for anti-GAPVD1 antibody: synthetic peptide directed towards the N terminal of human GAPVD1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAPVD1 |
|---|---|
| Gene Synonyms | DKFZP434C212 KIAA1521 MGC138847 MGC138848 RAP6 GAPEX5 |
| Gene Full Name | GTPase activating protein and VPS9 domains 1 |
| Gene Summary | GAPVD1 acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. It acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. It is also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. It has GEF activity for Rab5 and GAP activity for Ras. |
| Alternative Names | DKFZP434C212 KIAA1521 MGC138847 MGC138848 RAP6 GAPEX5 |
| Molecular Weight(MW) | 166kDa |
| Sequence | 1487 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
0.25ug/mlPositive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
