Application
Antibody used in WB on Human HepG2 at 0.25 ug/ml.Application
Antibody used in IHC on Human Brain.| Product Name | GAPVD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GAPVD1, DKFZP434C212, KIAA1521, MGC138847, MGC138848, RAP6, GAPEX5, GAPex-5 |
| Product description | GAPVD1 acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. It acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. It is also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. It has GEF activity for Rab5 and GAP activity for Ras.1) Hunker, C.M., (2006) Biochem. Biophys. Res. Commun. 340 (3), 967-975. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GAPVD1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| GAPVD1 antibody can be used for detection of GAPVD1 by ELISA at 1:312500. GAPVD1 antibody can be used for detection of GAPVD1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAPVD1 |
|---|---|
| Alternative Names | GAPVD1 DKFZP434C212 KIAA1521 MGC138847 MGC138848 RAP6 GAPEX5 GAPex-5 |
| Molecular Weight(MW) | 166 kDa |
Application
Antibody used in WB on Human HepG2 at 0.25 ug/ml.
Application
Antibody used in IHC on Human Brain.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | GAPVD1 antibody can be used for detection of GAPVD1 by ELISA at 1:312500. GAPVD1 antibody can be used for detection of GAPVD1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GAPVD1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
