Immunoblot
Lane 1: 50ug Mouse adipose lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-Rabbit-HRPSecondary Antibody Dilution: 1:10000Gene Name: NEDD4Submitted by: Libin Liu, Boston University.Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Product Name | Rabbit anti-NEDD4 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIAA0093, MGC176705, RPF1, NEDD4-1 |
| Immunogen | The immunogen for anti-NEDD4 antibody: synthetic peptide directed towards the middle region of human NEDD4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NEDD4 |
|---|---|
| Gene Synonyms | KIAA0093 MGC176705 RPF1 NEDD4-1 |
| Gene Full Name | Neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase |
| Gene Summary | NEDD4 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4 is involved in the budding of many viruses. |
| Alternative Names | KIAA0093 MGC176705 RPF1 NEDD4-1 |
| Molecular Weight(MW) | 104kDa |
| Sequence | 900 amino acids |
Immunoblot
Lane 1: 50ug Mouse adipose lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-Rabbit-HRPSecondary Antibody Dilution: 1:10000Gene Name: NEDD4Submitted by: Libin Liu, Boston University.
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
