Immunoblot
1: 40ng HIS-UBE2D1 protein2: 40ng HIS-UBE2D2 protein3: 40ng HIS-UBE2D3 protein4: 40ng HIS-UBE2D4 protein5: 40ng HIS-UBE2E1 protein6: 40ng HIS-UBE2E2 protein7: 40ng HIS-UBE2E3 protein8: 40ng HIS-UBE2K protein9: 40ng HIS-UBE2L3 protein10: 40ng HIS-UBE2N protein11: 40ng HIS-UBE2V1 protein12: 40ng HIS-UBE2V2 protein.Primary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:50,000Gene Name: UBE2E3Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HT1080 cell lysate| Product Name | Rabbit anti-UBE2E3 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBCH9, UbcM2 |
| Immunogen | The immunogen for anti-UBE2E3 antibody: synthetic peptide directed towards the N terminal of human UBE2E3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHumanMouseZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2E3 |
|---|---|
| Gene Synonyms | UBCH9 UbcM2 |
| Gene Full Name | Ubiquitin-conjugating enzyme E2E 3 |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| Alternative Names | UBCH9 UbcM2 |
| Molecular Weight(MW) | 23kDa |
| Sequence | 207 amino acids |
Immunoblot
1: 40ng HIS-UBE2D1 protein2: 40ng HIS-UBE2D2 protein3: 40ng HIS-UBE2D3 protein4: 40ng HIS-UBE2D4 protein5: 40ng HIS-UBE2E1 protein6: 40ng HIS-UBE2E2 protein7: 40ng HIS-UBE2E3 protein8: 40ng HIS-UBE2K protein9: 40ng HIS-UBE2L3 protein10: 40ng HIS-UBE2N protein11: 40ng HIS-UBE2V1 protein12: 40ng HIS-UBE2V2 protein.Primary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:50,000Gene Name: UBE2E3Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HT1080 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
