Western blot
Positive Control: Lane 1: 10ug MGRN1-GFP transfected HEK293T Lane 2: 10ug mut1MGRN1-GFP transfected HEK293T Lane 3: 10ug mut1MGRN2-GFP transfected HEK293T Lane 4: 10ug GFP transfected HEK293T Lane 5: 10ug IP for GFP using lysate from lane 1 Primary Antibody Dilution : 1:1000 Secondary Antibody : Goat anti rabbit-HRP Secondry Antibody Dilution : 1:50,000Submitted by: Teresa Gunn, McLaughin Research InstituteWestern blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: 721_B cell lysate| Product Name | Rabbit anti-MGRN1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIAA0544, RNF156 |
| Immunogen | The immunogen for anti-MGRN1 antibody: synthetic peptide directed towards the middle region of human MGRN1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGoatGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MGRN1 |
|---|---|
| Gene Synonyms | KIAA0544 RNF156 |
| Gene Full Name | Mahogunin ring finger 1, E3 ubiquitin protein ligase |
| Gene Summary | Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.[supplied by OMIM]. |
| Alternative Names | KIAA0544 RNF156 |
| Molecular Weight(MW) | 63kDa |
| Sequence | 576 amino acids |
Western blot
Positive Control: Lane 1: 10ug MGRN1-GFP transfected HEK293T Lane 2: 10ug mut1MGRN1-GFP transfected HEK293T Lane 3: 10ug mut1MGRN2-GFP transfected HEK293T Lane 4: 10ug GFP transfected HEK293T Lane 5: 10ug IP for GFP using lysate from lane 1 Primary Antibody Dilution : 1:1000 Secondary Antibody : Goat anti rabbit-HRP Secondry Antibody Dilution : 1:50,000Submitted by: Teresa Gunn, McLaughin Research Institute
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: 721_B cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
