Application
Western blot analysis of MGRN1 antibody (N-term) in T47D cell line lysates (35ug/lane). MGRN1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with MGRN1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
MGRN1 Antibody (N-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MGRN1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGRN1; KIAA0544; RNF156; E3 ubiquitin-protein ligase MGRN1; Mahogunin RING finger protein 1; RING finger protein 156 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0544 RNF156 |
|---|---|
| Alternative Names | MGRN1 KIAA0544 RNF156 E3 ubiquitin-protein ligase MGRN1 Mahogunin RING finger protein 1 RING finger protein 156 |
| Molecular Weight(MW) | 60753 Da |
| Function | E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to- lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination |
| Tissue Specificity | This MGRN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88~118 amino acids from the N-terminal region of human MGRN1. |
| Cellular Localization | Early endosome. Note=The endosomal localization is dependent on the interaction with TSG101 Isoform 2: Cytoplasm, cytosol. Nucleus. Note=Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli Isoform 4: Cytoplasm, cytosol. Cell membrane |
| Entrez Gene | 23295 |
|---|
Application
Western blot analysis of MGRN1 antibody (N-term) in T47D cell line lysates (35ug/lane). MGRN1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with MGRN1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
MGRN1 Antibody (N-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
