Application
Antibody used in WB on HEK293T at 1:1000 (Lane 1: 10ug MGRN1-GFP transfected HEK293T Lane 2: 10ug mut1MGRN1-GFP transfected HEK293T Lane 3: 10ug mut1MGRN2-GFP transfected HEK293T Lane 4: 10ug GFP transfected HEK293T Lane 5: 10ug IP for GFP using lysate from lane 1 ).Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Product Name | MGRN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGRN1, KIAA0544, RNF156 |
| Product description | Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.1) Kim, B.Y., (2007) Mol. Biol. Cell 18 (4), 1129-1142. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MGRN1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MGRN1 antibody can be used for detection of MGRN1 by ELISA at 1:12500. MGRN1 antibody can be used for detection of MGRN1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MGRN1 |
|---|---|
| Alternative Names | MGRN1 KIAA0544 RNF156 |
| Molecular Weight(MW) | 63 kDa |
Application
Antibody used in WB on HEK293T at 1:1000 (Lane 1: 10ug MGRN1-GFP transfected HEK293T Lane 2: 10ug mut1MGRN1-GFP transfected HEK293T Lane 3: 10ug mut1MGRN2-GFP transfected HEK293T Lane 4: 10ug GFP transfected HEK293T Lane 5: 10ug IP for GFP using lysate from lane 1 ).
Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | MGRN1 antibody can be used for detection of MGRN1 by ELISA at 1:12500. MGRN1 antibody can be used for detection of MGRN1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MGRN1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
