Immunohistochemical
BreastWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-CHFR polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ10796, FLJ33629, RNF116, RNF196 |
| Immunogen | The immunogen for anti-CHFR antibody: synthetic peptide directed towards the N terminal of human CHFR |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHFR |
|---|---|
| Gene Synonyms | FLJ10796 FLJ33629 RNF116 RNF196 |
| Gene Full Name | Checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase |
| Gene Summary | CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons. |
| Alternative Names | FLJ10796 FLJ33629 RNF116 RNF196 |
| Molecular Weight(MW) | 69kDa |
| Sequence | 623 amino acids |
Immunohistochemical
Breast
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
