Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate| Product Name | Rabbit anti-MARCH4 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MARCH-IV, MGC104908, RNF174 |
| Immunogen | The immunogen for anti-MARCH4 antibody: synthetic peptide directed towards the N terminal of human MARCH4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCH4 |
|---|---|
| Gene Synonyms | MARCH-IV MGC104908 RNF174 |
| Gene Full Name | Membrane-associated ring finger (C3HC4) 4, E3 ubiquitin protein ligase |
| Gene Summary | MARCH4 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. |
| Alternative Names | MARCH-IV MGC104908 RNF174 |
| Molecular Weight(MW) | 45kDa |
| Sequence | 410 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
