Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Product Name | MARCH4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCH4 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.1) Bartee, E., (2004) J. Virol. 78 (3), 1109-1120. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MARCH4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MARCH4 antibody can be used for detection of 40241 by ELISA at 1:312500. MARCH4 antibody can be used for detection of 40241 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCH4 |
|---|---|
| Alternative Names | MARCH4 MARCH-IV MGC104908 RNF174 |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | MARCH4 antibody can be used for detection of 40241 by ELISA at 1:312500. MARCH4 antibody can be used for detection of 40241 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MARCH4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
