Immunohistochemistry
Immunohistochemistry with Human Liver cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-HSD11B1 antibodyWestern blot
1 ug/mlPositive Control: Fetal liver cell lysate| Product Name | Rabbit anti-HSD11B1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 11-DH, 11-beta-HSD1, HDL, HSD11, HSD11B, HSD11L, MGC13539, SDR26C1 |
| Immunogen | The immunogen for anti-HSD11B1 antibody: synthetic peptide directed towards the N terminal of human HSD11B1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSD11B1 |
|---|---|
| Gene Synonyms | 11-DH 11-beta-HSD1 HDL HSD11 HSD11B HSD11L MGC13539 SDR26C1 |
| Gene Full Name | Hydroxysteroid (11-beta) dehydrogenase 1 |
| Gene Summary | HSD11B1 is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, HSD11B1 can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children.The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene. |
| Alternative Names | 11-DH 11-beta-HSD1 HDL HSD11 HSD11B HSD11L MGC13539 SDR26C1 |
| Molecular Weight(MW) | 32kDa |
| Sequence | 292 amino acids |
Immunohistochemistry
Immunohistochemistry with Human Liver cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-HSD11B1 antibody
Western blot
1 ug/mlPositive Control: Fetal liver cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
