Western blot
Sample Type: 1. Hamster CHO K1 cells (20ug)2. HeLa skin cells(20ug)3. HEK273 cells (20ug)4. Human Skin Fibroblats (100ug)Primary Dilution: 1:800Secondary Antibody: Clean-Blot IP detection Reagent and KitSecondary Dilution: 1:2000Image Submitted By: V. Skopova, M. ZikanovaInstitute of Inherited Metabolic Disorders, Charles University in Prague and General Hopspital in PragueWestern blot
1.25ug/mlPositive Control: HepG2 cell lysate| Product Name | Rabbit anti-ATIC polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AICAR, AICARFT, IMPCHASE, PURH |
| Immunogen | The immunogen for anti-ATIC antibody: synthetic peptide directed towards the N terminal of human ATIC |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHorseHumanPigRabbitZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ATIC |
|---|---|
| Gene Synonyms | AICAR AICARFT IMPCHASE PURH |
| Gene Full Name | 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase |
| Gene Summary | ATIC is a bifunctional protein requiring dimerization for transformylase activity. |
| Alternative Names | AICAR AICARFT IMPCHASE PURH |
| Molecular Weight(MW) | 64kDa |
| Sequence | 592 amino acids |
Western blot
Sample Type: 1. Hamster CHO K1 cells (20ug)2. HeLa skin cells(20ug)3. HEK273 cells (20ug)4. Human Skin Fibroblats (100ug)Primary Dilution: 1:800Secondary Antibody: Clean-Blot IP detection Reagent and KitSecondary Dilution: 1:2000Image Submitted By: V. Skopova, M. ZikanovaInstitute of Inherited Metabolic Disorders, Charles University in Prague and General Hopspital in Prague
Western blot
1.25ug/mlPositive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
