Immunoblot
1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: CADSubmitted by: Dr. Pankaj Singh, UNMC, Omaha, NEWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate| Product Name | Rabbit anti-CAD polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-CAD antibody: synthetic peptide directed towards the N terminal of human CAD |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAD |
|---|---|
| Gene Synonyms | - |
| Gene Full Name | Carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase |
| Gene Summary | CAD is a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Molecular Weight(MW) | 243kDa |
| Sequence | 2225 amino acids |
Immunoblot
1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: CADSubmitted by: Dr. Pankaj Singh, UNMC, Omaha, NE
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
