Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :WBP11Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :WBP11Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
Host: Rabbit Target Name: WBP11 Sample Tissue: Hela Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: WBP11 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: WBP11 Sample Tissue: Jurkat Antibody Dilution: 1.0ug/mlImmunoblot
1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: WBP11Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Hela cell lysate| Product Name | Rabbit anti-WBP11 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp779M1063, NPWBP, SIPP1, WBP-11 |
| Immunogen | The immunogen for anti-WBP11 antibody: synthetic peptide directed towards the N terminal of human WBP11 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHorseHumanMouseRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WBP11 |
|---|---|
| Gene Synonyms | DKFZp779M1063 NPWBP SIPP1 WBP-11 |
| Gene Full Name | WW domain binding protein 11 |
| Gene Summary | WBP11 is a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. WBP11has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus.This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus. |
| Alternative Names | DKFZp779M1063 NPWBP SIPP1 WBP-11 |
| Molecular Weight(MW) | 70kDa |
| Sequence | 641 amino acids |
Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :WBP11Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :WBP11Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
Host: Rabbit Target Name: WBP11 Sample Tissue: Hela Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: WBP11 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: WBP11 Sample Tissue: Jurkat Antibody Dilution: 1.0ug/ml
Immunoblot
1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: WBP11Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
