Application
Antibody used in WB on Human, Mouse at 2 ug/ml (Lane 1. Human NT-2 cells (60ug) lysate Lane 2. Mouse WT brain extract (80ug)).Application
Antibody used in IP on Mouse brain at 1:500.Application
Antibody used in WB on Human Jurkat 1 ug/ml.Application
Antibody used in WB on Human HeLa cells at 0.2-1 ug/ml.| Product Name | WBP11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WBP11, DKFZp779M1063, NPWBP, SIPP1, WBP-11 |
| Product description | WBP11 is a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. WBP11has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus.This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus.1) Olsen, J.V., (2006) Cell 127 (3), 635-648. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human WBP11. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WBP11 antibody can be used for detection of WBP11 by ELISA at 1:12500. WBP11 antibody can be used for detection of WBP11 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WBP11 |
|---|---|
| Alternative Names | WBP11 DKFZp779M1063 NPWBP SIPP1 WBP-11 |
| Molecular Weight(MW) | 70 kDa |
Application
Antibody used in WB on Human, Mouse at 2 ug/ml (Lane 1. Human NT-2 cells (60ug) lysate Lane 2. Mouse WT brain extract (80ug)).
Application
Antibody used in IP on Mouse brain at 1:500.
Application
Antibody used in WB on Human Jurkat 1 ug/ml.
Application
Antibody used in WB on Human HeLa cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | WBP11 antibody can be used for detection of WBP11 by ELISA at 1:12500. WBP11 antibody can be used for detection of WBP11 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store WBP11 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
