Immunoblot
1: 30ug HeLa lysate, 2: 30ug HFF lysate, 3: 30ug U2OS lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: ING1Submitted by: Dr. Hyung Suk Oh, Knipe Lab, Harvard Medical School| Product Name | Rabbit anti-ING1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a |
| Immunogen | The immunogen for anti-ING1 antibody: synthetic peptide directed towards the C terminal of human ING1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ING1 |
|---|---|
| Gene Synonyms | p24ING1c p33 p33ING1 p33ING1b p47 p47ING1a |
| Gene Full Name | Inhibitor of growth family, member 1 |
| Gene Summary | ING1 is a tumor suppressor protein that can induce cell growth arrest and apoptosis. The protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of ING1 gene have been detected in various cancers.This gene encodes a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. |
| Alternative Names | p24ING1c p33 p33ING1 p33ING1b p47 p47ING1a |
| Molecular Weight(MW) | 32kDa |
| Sequence | 279 amino acids |
Immunoblot
1: 30ug HeLa lysate, 2: 30ug HFF lysate, 3: 30ug U2OS lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: ING1Submitted by: Dr. Hyung Suk Oh, Knipe Lab, Harvard Medical School| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
