Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 30ug HeLa lysate, Lane 2: 30ug HFF lysate, Lane 3: 30ug U2OS lysate).| Product Name | ING1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ING1, p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a |
| Product description | ING1 is a tumor suppressor protein that can induce cell growth arrest and apoptosis. The protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of ING1 gene have been detected in various cancers.This gene encodes a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.1) Ahmed, I.A., (2008) Colorectal Dis 10 (4), 344-351. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ING1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ING1 antibody can be used for detection of ING1 by ELISA at 1:62500. ING1 antibody can be used for detection of ING1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ING1 |
|---|---|
| Alternative Names | ING1 p24ING1c p33 p33ING1 p33ING1b p47 p47ING1a |
| Molecular Weight(MW) | 32 kDa |
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 30ug HeLa lysate, Lane 2: 30ug HFF lysate, Lane 3: 30ug U2OS lysate).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ING1 antibody can be used for detection of ING1 by ELISA at 1:62500. ING1 antibody can be used for detection of ING1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ING1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
