Western blot
Sample Type: 1. Human NT-2 cells (60ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlPositive Control: Hela cell lysate| Product Name | Rabbit anti-UBE2F polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC18120, NCE2 |
| Immunogen | The immunogen for anti-UBE2F antibody: synthetic peptide directed towards the N terminal of human UBE2F |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2F |
|---|---|
| Gene Synonyms | MGC18120 NCE2 |
| Gene Full Name | Ubiquitin-conjugating enzyme E2F (putative) |
| Gene Summary | UBE2F belongs to the ubiquitin-conjugating enzyme family. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. |
| Alternative Names | MGC18120 NCE2 |
| Molecular Weight(MW) | 21kDa |
| Sequence | 185 amino acids |
Western blot
Sample Type: 1. Human NT-2 cells (60ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical Science
Western blot
0.2-1 ug/mlPositive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
