Application
Antibody used in WB on Mouse Brain at 2 ug/ml.Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Brain at: 1:50.| Product Name | UBE2F Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2F, MGC18120, NCE2 |
| Product description | UBE2F belongs to the ubiquitin-conjugating enzyme family. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.1) Hillier, L.W., (2005) Nature 434 (7034), 724-731. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2F. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| UBE2F antibody can be used for detection of UBE2F by ELISA at 1:312500. UBE2F antibody can be used for detection of UBE2F by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2F |
|---|---|
| Alternative Names | UBE2F MGC18120 NCE2 |
| Molecular Weight(MW) | 21 kDa |
Application
Antibody used in WB on Mouse Brain at 2 ug/ml.
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Brain at: 1:50.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | UBE2F antibody can be used for detection of UBE2F by ELISA at 1:312500. UBE2F antibody can be used for detection of UBE2F by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store UBE2F antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
