Immunoblot
1: 30ug HEK293T cell lysate, 2: 30ug 3FLAG-hFEM1B transfected HEK293T cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:8000Gene Name: FEM1BSubmitted by: AnonymousWestern blot
0.2-1 ug/mlPositive Control: 721_B cell lysate| Product Name | Rabbit anti-FEM1B polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp451E0710, FIAA, F1A-ALPHA |
| Immunogen | The immunogen for anti-FEM1B antibody: synthetic peptide directed towards the middle region of human FEM1B |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FEM1B |
|---|---|
| Gene Synonyms | DKFZp451E0710 FIAA F1A-ALPHA |
| Gene Full Name | Fem-1 homolog b (C. elegans) |
| Gene Summary | FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. It involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. FEM1B also involved in glucose homeostasis in pancreatic islet. |
| Alternative Names | DKFZp451E0710 FIAA F1A-ALPHA |
| Molecular Weight(MW) | 69kDa |
| Sequence | 627 amino acids |
Immunoblot
1: 30ug HEK293T cell lysate, 2: 30ug 3FLAG-hFEM1B transfected HEK293T cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:8000Gene Name: FEM1BSubmitted by: Anonymous
Western blot
0.2-1 ug/mlPositive Control: 721_B cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
