Application
Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 μg/mL.Application
Immunofluorescence of F1A alpha in mouse liver tissue with F1A alpha antibody at 20 μg/ml.Application
Immunohistochemistry of F1Aα in mouse liver tissue with F1Aα antibody at 5 μg/mL.Application
Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 μg/mL.| Product Name | F1A alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | F1A alpha Antibody: F1AA, F1A-ALPHA, FEM1-beta, F1AA, KIAA0396, Protein fem-1 homolog B, FEM1b |
| Product description | F1A alpha Antibody: Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1β. F1Aalpha/FEM1β is the homologue of C. elegans sex determining protein FEM-1. FEM-1/F1Aalpha is cleaved by CED-3 and caspase. FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues.1) Chan SL, Tan KO, Zhang L, Yee KS , Ronca F, Chan MY, Yu VC. F1Aα, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis. J Biol Chem. 1999;274(45):32461-8. |
| Immunogen | F1A antibody was raised against a 14 amino acid peptide near the carboxy terminus of human F1A. The immunogen is located within the last 50 amino acids of F1A alpha. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| F1Ab antibody can be used for detection of F1Ab by Western blot at 1 μg/mL. An approximately 70 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FEM1B |
|---|---|
| Alternative Names | F1A alpha Antibody: F1AA F1A-ALPHA FEM1-beta F1AA KIAA0396 Protein fem-1 homolog B FEM1b |
| Molecular Weight(MW) | 70 kDa |
| Sequence Similarities | 2) Cat. No. 1464 - Rat Liver Tissue Lysate |
Application
Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 μg/mL.
Application
Immunofluorescence of F1A alpha in mouse liver tissue with F1A alpha antibody at 20 μg/ml.
Application
Immunohistochemistry of F1Aα in mouse liver tissue with F1Aα antibody at 5 μg/mL.
Application
Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | F1Ab antibody can be used for detection of F1Ab by Western blot at 1 μg/mL. An approximately 70 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | F1A alpha antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | F1A alpha Antibody is supplied in PBS containing 0.02% sodium azide. |
