Application
Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.Application
Antibody used in WB on Human HEK293T at: 1:1000 (Lane 1: 30ug HEK293T cell lysate, Lane 2: 30ug 3FLAG-hFEM1B transfected HEK293T cell lysate).| Product Name | FEM1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. It involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. FEM1B also involved in glucose homeostasis in pancreatic islet.1) Kamura, T., (2004) Genes Dev. 18 (24), 3055-3065. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FEM1B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| FEM1B antibody can be used for detection of FEM1B by ELISA at 1:1562500. FEM1B antibody can be used for detection of FEM1B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FEM1B |
|---|---|
| Alternative Names | FEM1B DKFZp451E0710 FIAA F1AA F1A-ALPHA FEM1-beta |
| Molecular Weight(MW) | 69 kDa |
Application
Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.
Application
Antibody used in WB on Human HEK293T at: 1:1000 (Lane 1: 30ug HEK293T cell lysate, Lane 2: 30ug 3FLAG-hFEM1B transfected HEK293T cell lysate).| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | FEM1B antibody can be used for detection of FEM1B by ELISA at 1:1562500. FEM1B antibody can be used for detection of FEM1B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store FEM1B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
