Western blot
Sample Type: 1. Human 293FT cell line (15ug)2. Murine IMCD3 cell line (15ug)3. Human RPE cell line (15ug)4. Bovine retina lysate cleared at 100,000Xg for 2 hours (15ug)5. Mouse Brain Lysate (15ug)Primary Dilution: 1:1000Secondary Anditbody: goat anti-rabbit HRPScondary Dilution: 1:10,000Image Submitted By: Gerald LiewStanford UniversityWestern blot
0.2-1 ug/mlPositive Control: 721_B cell lysate| Product Name | Rabbit anti-IFT122 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPG, WDR10, WDR10p, WDR140, CED |
| Immunogen | The immunogen for anti-IFT122 antibody: synthetic peptide directed towards the C terminal of human IFT122 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFT122 |
|---|---|
| Gene Synonyms | SPG WDR10 WDR10p WDR140 CED |
| Gene Full Name | Intraflagellar transport 122 homolog (Chlamydomonas) |
| Gene Summary | IFT122 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. IFT122 contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation.This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This cytoplasmic protein contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Alternative Names | SPG WDR10 WDR10p WDR140 CED |
| Molecular Weight(MW) | 129kDa |
| Sequence | 1131 amino acids |
Western blot
Sample Type: 1. Human 293FT cell line (15ug)2. Murine IMCD3 cell line (15ug)3. Human RPE cell line (15ug)4. Bovine retina lysate cleared at 100,000Xg for 2 hours (15ug)5. Mouse Brain Lysate (15ug)Primary Dilution: 1:1000Secondary Anditbody: goat anti-rabbit HRPScondary Dilution: 1:10,000Image Submitted By: Gerald LiewStanford University
Western blot
0.2-1 ug/mlPositive Control: 721_B cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
