Immunohistochemical
Paraffin embedded brain, cerebellum tissue, tested with an antibody Dilution of 5 ug/ml.Western blot
Host: Rabbit Target Name: ARF6 Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: ARF6 Sample Tissue: Human Adult Placenta Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: ARF6 Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: ARF6 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: ARF6 Sample Tissue: Jurkat Antibody Dilution: 1.0ug/mlWestern blot
0.2-1 ug/mlPositive Control: MCF7 cell lysate| Product Name | Rabbit anti-ARF6 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp564M0264 |
| Immunogen | The immunogen for anti-ARF6 antibody: synthetic peptide directed towards the middle region of human ARF6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRabbitRatSheepZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARF6 |
|---|---|
| Gene Synonyms | DKFZp564M0264 |
| Gene Full Name | ADP-ribosylation factor 6 |
| Gene Summary | ARF6 is a member of the human ARF family, which is part of the RAS superfamily. They are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF6 is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | DKFZp564M0264 |
| Molecular Weight(MW) | 20kDa |
| Sequence | 175 amino acids |
Immunohistochemical
Paraffin embedded brain, cerebellum tissue, tested with an antibody Dilution of 5 ug/ml.
Western blot
Host: Rabbit Target Name: ARF6 Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: ARF6 Sample Tissue: Human Adult Placenta Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: ARF6 Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: ARF6 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: ARF6 Sample Tissue: Jurkat Antibody Dilution: 1.0ug/ml
Western blot
0.2-1 ug/mlPositive Control: MCF7 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
