Application
Western blot analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 μg/ml.Application
Immunohistochemistry of ARF6 in human kidney tissue with ARF6 antibody at 5 μg/mL.Application
Immunofluorescence of ARF6 in human kidney tissue with ARF6 antibody at 20 μg/mL.| Product Name | ARF6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF6 Antibody: ADP-ribosylation factor 6 |
| Product description | The ADP-ribosylation factor 6 (ARF6) is a member of the ARF family, which is part of the RAS superfamily (1). The ARF proteins are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D (2). The product of this gene is localized to the plasma membrane (3), and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling (4).1) Graves RA, Tontonoz P, and Spiegelman BM. Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression. Mol. Cell Biol. 1992; 12:1202-8. |
| Immunogen | ARF6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ARF6. The immunogen is located within the last 50 amino acids of ARF6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ARF6 antibody can be used for detection of ARF6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARF6 |
|---|---|
| Alternative Names | ARF6 Antibody: ADP-ribosylation factor 6 |
| Molecular Weight(MW) | Predicted: 19 kDa Observed: 18 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | ARF6 antibody is human, mouse and rat reactive. |
Application
Western blot analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 μg/ml.
Application
Immunohistochemistry of ARF6 in human kidney tissue with ARF6 antibody at 5 μg/mL.
Application
Immunofluorescence of ARF6 in human kidney tissue with ARF6 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | ARF6 antibody can be used for detection of ARF6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ARF6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ARF6 antibody is supplied in PBS containing 0.02% sodium azide. |
