Application
Antibody used in IHC on Human Brain at of 5 ug/ml.Application
Antibody used in WB on Human MCF7 cells at 0.2-1 ug/ml.| Product Name | ARF6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF6, DKFZp564M0264, |
| Product description | ARF6 is a member of the human ARF family, which is part of the RAS superfamily. They are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF6 is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Karim, Z.A., (2008) J. Biol. Chem. 283 (18), 11995-12003. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ARF6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ARF6 antibody can be used for detection of ARF6 by ELISA at 1:62500. ARF6 antibody can be used for detection of ARF6 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DrosophilaHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARF6 |
|---|---|
| Alternative Names | ARF6 DKFZp564M0264 |
| Molecular Weight(MW) | 20 kDa |
Application
Antibody used in IHC on Human Brain at of 5 ug/ml.
Application
Antibody used in WB on Human MCF7 cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | ARF6 antibody can be used for detection of ARF6 by ELISA at 1:62500. ARF6 antibody can be used for detection of ARF6 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ARF6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
