Immunofluorescence
SENP3 antibody - N-terminal region in HeLa cells using ImmunohistochemistryWestern blot
Sample Type: 1. HeLa cell extract (200ug)2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME*HA-S2-VME-SENP3Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, PortugalWestern blot
Sample Type: 1. HeLa cell extract (200ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, PortugalWestern blot
Sample Type: 1. Rat Liver cells (200ug)2. Rat Brain cells (200ug)3. Rat Testis cells (200ug) 4. Rat Kidney cells (200ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal See Customer Feedback tab for detailed information.Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysate| Product Name | Rabbit anti-SENP3 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZP586K0919, DKFZp762A152, SMT3IP1, SSP3 |
| Immunogen | The immunogen for anti-SENP3 antibody: synthetic peptide directed towards the N terminal of human SENP3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SENP3 |
|---|---|
| Gene Synonyms | DKFZP586K0919 DKFZp762A152 SMT3IP1 SSP3 |
| Gene Full Name | SUMO1/sentrin/SMT3 specific peptidase 3 |
| Gene Summary | SENP3 is a protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. SENP3 deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. |
| Alternative Names | DKFZP586K0919 DKFZp762A152 SMT3IP1 SSP3 |
| Molecular Weight(MW) | 65kDa |
| Sequence | 574 amino acids |
Immunofluorescence
SENP3 antibody - N-terminal region in HeLa cells using Immunohistochemistry
Western blot
Sample Type: 1. HeLa cell extract (200ug)2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME*HA-S2-VME-SENP3Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal
Western blot
Sample Type: 1. HeLa cell extract (200ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal
Western blot
Sample Type: 1. Rat Liver cells (200ug)2. Rat Brain cells (200ug)3. Rat Testis cells (200ug) 4. Rat Kidney cells (200ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal See Customer Feedback tab for detailed information.
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
