WB
Various lysates were subjected to SDS PAGE followed by western blot with SENP3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using SENP3 antibody at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-SENP3 (IP:4ug; Detection:1:500) with Y79 cells lysate 2000ug.| Product Name | SENP3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SENP3 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:2000 IHC:1:250-1:1000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanRat |
| Concentration | 0.45mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SENP3 |
|---|---|
| Gene Synonyms | SSP3 Ulp1 SMT3IP1 |
| Gene Full Name | SUMO specific peptidase 3 |
| Gene Summary | The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP3, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates (Di Bacco et al., 2006 [PubMed 16738315]).[supplied by OMIM, Jun 2009] |
| Alternative Names | SUMO-1-specific protease 3 SSP3 SMT3IP1 Sentrin-specific protease 3 Sentrin/SUMO-specific protease SENP3 |
| Molecular Weight(MW) | 65-70 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SENP3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using SENP3 antibody at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-SENP3 (IP:4ug; Detection:1:500) with Y79 cells lysate 2000ug.| Application Notes | WB:1:500-1:2000 IHC:1:250-1:1000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
