Western blot
Host: RabbitTarget Name: SLC13A3 Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: NOP56 Sample Tissue: Human Fetal Brain Antibody Dilution: 1.0ug/mlWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-PRAME polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAPE, OIP4, CT130 |
| Immunogen | The immunogen for anti-PRAME antibody: synthetic peptide directed towards the N terminal of human PRAME |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRAME |
|---|---|
| Gene Synonyms | MAPE OIP4 CT130 |
| Gene Full Name | Preferentially expressed antigen in melanoma |
| Gene Summary | PRAME functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. PRAME prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene. |
| Alternative Names | MAPE OIP4 CT130 |
| Molecular Weight(MW) | 56kDa |
| Sequence | 509 amino acids |
Western blot
Host: RabbitTarget Name: SLC13A3 Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: NOP56 Sample Tissue: Human Fetal Brain Antibody Dilution: 1.0ug/ml
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
