Western blot
Lane 1: 20ug siRUVBL1 transfected human H1299 cells Lane 2: 20ug untransfected human H1299 cells Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:3000 Color/Signal Descriptions:RAC1 Gene Name:Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey. Submitted by:Western blot
0.2-1 ug/mlPositive Control: HepG2 cell lysate| Product Name | Rabbit anti-RAC1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC111543, MIG5, TC-25, p21-Rac1, Rac-1 |
| Immunogen | The immunogen for anti-RAC1 antibody: synthetic peptide directed towards the middle region of human RAC1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMouseRabbitRatSheepZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAC1 |
|---|---|
| Gene Synonyms | MGC111543 MIG5 TC-25 p21-Rac1 Rac-1 |
| Gene Full Name | Ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) |
| Gene Summary | The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | MGC111543 MIG5 TC-25 p21-Rac1 Rac-1 |
| Molecular Weight(MW) | 21kDa |
| Sequence | 192 amino acids |
Western blot
Lane 1: 20ug siRUVBL1 transfected human H1299 cells Lane 2: 20ug untransfected human H1299 cells Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:3000 Color/Signal Descriptions:RAC1 Gene Name:Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey. Submitted by:
Western blot
0.2-1 ug/mlPositive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
