WB
Various lysates were subjected to SDS PAGE followed by western blot with Rac1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RAC1 Antibody at dilution of 1:400 (under 40x lens).ICC/IF
Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using Rac1 antibody at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).IP
IP result of anti-RAC1 (IP:3ug; Detection: 1:700) with HL-60 cells lysate 4mg.| Product Name | RAC1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human RAC1 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:5000 IHC:1:50-1:500 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAC1 |
|---|---|
| Alternative Names | RAC1 MIG5 p21-Rac1 Ras-like protein TC25 TC25 TC-25 Rac-1 |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Rac1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RAC1 Antibody at dilution of 1:400 (under 40x lens).
ICC/IF
Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using Rac1 antibody at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).
IP
IP result of anti-RAC1 (IP:3ug; Detection: 1:700) with HL-60 cells lysate 4mg.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:500 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | RAC1 Monoclonal antibody may be stored at Long term: -20˚C; short term: +4˚C |
| Storage Buffer | Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide. |
