Application
Western blot analysis of lysed extracts from 293 and HeLa cells using Rac1 (Ab-71).Application
Immunofluorescence staining of methanol-fixed HeLa cells using Rac1 (Ab-71).| Product Name | Rac1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rac1 encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.1) Aoki K, et al. (2005) Mol Biol Cell; 16 (5): 2207–2217. Kwon T, et al. (2000) J Biol Chem; 275 (1): 423-428 |
| Immunogen | Rac1 (Ab-71) antibody was raised against a peptide sequence around aa.69~73 (P-L-S-Y-P) derived from Human Rac1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAC1 |
|---|---|
| Alternative Names | MIG5 Rac-1 TC-25 p21-Rac1 Ras-like protein TC25 Ras-related C3 botulinum toxin substrate 1 |
| Molecular Weight(MW) | 21 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Rac1 protein. |
| Entrez Gene | 5879 |
|---|---|
| Protein Accession | NP_008839.2 |
Application
Western blot analysis of lysed extracts from 293 and HeLa cells using Rac1 (Ab-71).
Application
Immunofluorescence staining of methanol-fixed HeLa cells using Rac1 (Ab-71).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
