Western blot
GRB2 antibody - N-terminal region validated by WB using 1. and 2. Human Cystic Fibrosis Bronchial Epithelial Cells (35ug) Primary Dilution: 1:500Secondary Anitbody: goat anti-rabbit-HRPSecondary Dilution: 1:5000Image Submitted By: Dr. Haouaria BalghiMcGill UniversityWestern blot
Sample Type: 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical ScienceWestern blot
GRB2 antibody - N-terminal region validated by WB using 721_B cell Lysate at 1ug/ml.| Product Name | Rabbit anti-GRB2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GRB2 |
|---|---|
| Gene Synonyms | ASH EGFRBP-GRB2 Grb3-3 MST084 MSTP084 NCKAP2 |
| Gene Full Name | Growth factor receptor-bound protein 2 |
| Gene Summary | The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | ASH EGFRBP-GRB2 Grb3-3 MST084 MSTP084 NCKAP2 |
| Molecular Weight(MW) | 24kDa |
| Sequence | 217 amino acids |
Western blot
GRB2 antibody - N-terminal region validated by WB using 1. and 2. Human Cystic Fibrosis Bronchial Epithelial Cells (35ug) Primary Dilution: 1:500Secondary Anitbody: goat anti-rabbit-HRPSecondary Dilution: 1:5000Image Submitted By: Dr. Haouaria BalghiMcGill University
Western blot
Sample Type: 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical Science
Western blot
GRB2 antibody - N-terminal region validated by WB using 721_B cell Lysate at 1ug/ml.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
