WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GRB2 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human colon carcinoma was stained with anti-GRB2 Rabbit antibody.| Product Name | GRB2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GRB2 |
|---|---|
| Gene Synonyms | ASH Grb3-3 MST084 NCKAP2 MSTP084 EGFRBP-GRB2 |
| Gene Full Name | growth factor receptor bound protein 2 |
| Gene Summary | The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names |
GRB2 ASH Growth factor receptor-bound protein 2 Adapter protein GRB2 Protein Ash SH2/SH3 adapter GRB2 |
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Nucleus . Cytoplasm . Endosome . Golgi apparatus . |
| SwissProt ID | P62993 |
|---|
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GRB2 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human colon carcinoma was stained with anti-GRB2 Rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
